Coding

Part:BBa_K3425100:Design

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-07)


DNA Binding Domain-caffeine nanobody fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
    Illegal BglII site found at 930
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 622
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Finding dimerizing nanobodies might be more complicated than expected. It's good practice to confirm that novel fusion proteins expressing a known nanobody are indeed expressing a correctly folded protein, with binding capability.


Source

Chang, H. J., Mayonove, P., Zavala, A., De Visch, A., Minard, P., Cohen-Gonsaud, M., & Bonnet, J. (2018). A modular receptor platform to expand the sensing repertoire of bacteria. ACS synthetic biology, 7(1), 166-175.

References